human placenta cell line  (ATCC)

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Lactate dehydrogenase (LDH) release in BeWo cells incubated for 24 h with increasing concentrations of deoxynivalenol (DON), zearalenone (ZEN) and T-2 toxin. Data are representative of three independent experiments, each performed in triplicate, and expressed as the mean ± SEM. Different lowercase letters denote significant differences among the treatments ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Incubation

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of deoxynivalenol (DON), T-2 toxin, and zearalenone (ZEN) exposure on the integrity of the BeWo cell monolayer. The changes in ( A ) transepithelial electrical resistance (TEER) and ( B ) fluorescein isothiocyanate-dextran (FITC-D) flux from apical to basolateral compartment after 24 h exposure to mycotoxins at the apical side are shown. Data are expressed as the mean ± SEM of three independent experiments, each performed in triplicate. Different lowercase letters denote significant differences among groups. ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques:

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of deoxynivalenol (DON) exposure on mRNA levels of junctional proteins in BeWo cells. The mRNA expression of occludin (OCLD), zonula occludens protein-1 (ZO-1), claudin (CLDN)-3 and 4, and E-cadherin (E-cad) in BeWo cells after 24 h exposure to different concentrations of DON. Data are expressed as the mean ± SEM of three independent experiments, each performed in triplicate. Different lowercase letters denote significant differences among groups ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Expressing

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of zearalenone (ZEN) exposure on mRNA levels of junctional proteins in BeWo cells. The mRNA expression of occludin (OCLD), zonula occludens protein-1 (ZO-1), claudin (CLDN)-3 and 4, and E-cadherin (E-cad) in BeWo cells after 24 h exposure to different concentrations of ZEN. Data are expressed as the mean ± SEM of three independent experiments, each performed in triplicate. Different lowercase letters denote significant differences among groups ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Expressing

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of T-2 toxin exposure on mRNA levels of junctional proteins in BeWo cells. The mRNA expression of occludin (OCLD), zonula occludens protein-1 (ZO-1), claudin (CLDN)-3 and 4, and E-cadherin (E-cad) in BeWo cells after 24 h exposure to different concentrations of T-2 toxin. Data are expressed as the mean ± SEM of three independent experiments, each performed in triplicate. Different lowercase letters denote significant differences among groups ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Expressing

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of deoxynivalenol (DON) exposure on junctional proteins in BeWo cells. The protein expression of occludin (OCLD), zonula occludens protein-1 (ZO-1), claudin (CLDN)-3 and 4, and E-cadherin (E-cad) in BeWo cells after 24 h exposure to different concentrations of DON. Data are expressed as the mean ± SEM of three independent experiments, each performed in triplicate. Different lowercase letters denote significant differences among groups ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Expressing

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of zearalenone (ZEN) exposure on junctional proteins in BeWo cells. The protein expression of occludin (OCLD), zonula occludens protein-1 (ZO-1), claudin (CLDN)-3 and 4, and E-cadherin (E-cad) in BeWo cells after 24 h exposure to different concentrations of ZEN. Data are expressed as the mean ± SEM of three independent experiments, each performed in triplicate. Different lowercase letters denote significant differences among groups ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Expressing

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of T-2 toxin exposure on junctional proteins in BeWo cells. The protein expression of occludin (OCLD), zonula occludens protein-1 (ZO-1), claudin (CLDN)-3 and 4, and E-cadherin (E-cad) in BeWo cells after 24 h exposure to different concentrations of T-2 toxin. Data are expressed as the mean ± SEM of three independent experiments, each performed in triplicate. Different lowercase letters denote significant differences among groups ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Expressing

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of deoxynivalenol (DON), T-2 toxin, and zearalenone (ZEN) exposure on ( A ) occludin (OCLD) and ( B ) claudin (CLDN)-4 localization in BeWo cells, visualized by immunofluorescence staining (400× magnification). Scale bars represent 50 μm.

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Immunofluorescence, Staining

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of deoxynivalenol (DON), T-2 toxin, and zearalenone (ZEN) exposure on ( A ) occludens protein-1 (ZO-1) and ( B ) E-cadherin localization in BeWo cells, visualized by immunofluorescence staining (400× magnification). Scale bars represent 50 μm.

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Immunofluorescence, Staining

Journal: Toxins

Article Title: Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line

doi: 10.3390/toxins11110665

Figure Lengend Snippet: Effects of mycotoxin exposure on IL-6 mRNA expression and IL-6 secretion in BeWo cells. BeWo cells were incubated for 24 h with increasing concentrations of deoxynivalenol (DON), zearalenone (ZEN), and T-2 toxin and the IL-6 mRNA expression ( A ) and secretion ( B ) were measured. Data are expressed as the mean ± SEM of three independent experiments, each performed in triplicate. Different lowercase letters denote significant differences among groups ( p < 0.05).

Article Snippet: The human placenta choriocarcinoma (BeWo) cell line was obtained from the American Type Culture Collection (ATCC-CCL-98, Rockville, MD, USA; passages 11–45) and was cultured in culture flasks (Falcon, VWR International, Strasbourg, France) in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; Gibco, Thermo Fisher Scientific, Wilmington, DE, USA), supplemented with 10% fetal bovine serum (Perbio Science, Brebières, France), and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) mixture.

Techniques: Expressing, Incubation